10 mL Tube format

The MCSG Suite has been developed at the Midwest Center for Structural Genomics. As part of the Protein Structure Initiative, MCSG has been instrumental in the development of rapid, integrated and cost-effective methods for protein structure determination via X-ray crystallography. 

 

Through extensive crystallization screening of more than 40,000 diverse proteins from both prokaryotic and eukaryotic organisms, researchers at the MCSG have derived the most up-to-date set of crystallization conditions for initial screening. These non-redundant conditions constitute the broadest coverage of crystallization space of any commercially available screen and are supported by a decade of experimental analysis and validation.


Features:

  • 384 Crystallization conditions, optimized  through high throughput validation
  • Derived from trials of more than 40,000 diverse proteins at the Midwest Center for Structural Genomics
  • Broad coverage of crystallization space
  • Derived from crystallization of from both prokaryotic and eukaryotic organisms

The complete MCSG suite (384 conditions) comprises of 4 screens of 96 unique conditions that are available either as deep-well blocks (1.7 mL per well) or tubes (10 ml).  Conditions 1-288 are organized in order of decreasing crystal productivity in screens MCSG-1, MCSG-2 and MCSG-3.  Thus MCSG-1:A1 condition has yielded the greatest number of unique crystals for all targets screened with the suite predecessors.  The conditions that comprise MCSG-4 have been rationally selected to systematically sample regions of crystallization space for proteins requiring additional screening.

                                                            

How can Microlytic screens improve my current vapor diffusion pipeline?

The last two decades have seen a rapid expansion in the number of commercially available screens, each with distinguishing design rationales.  In most laboratories, multiple crystallization screens are kept onhand without a clearly defined workflow to establish the order in which screens should be used, guidance in differences of design rational or coverage of crystallization space, and without an easy reference to determine the level of redundancy between two reagent sets.  As a result, much of the crystallization screening becomes highly redundant, increasing the protein requirements without a commensurate increase in crystallization outcome. 

 

To support preferred vapor diffusion workflows, we introduced the MCSG Crystallization Suite, developed at the Midwest Center for Structural Genomics at Argonne National Laboratories through Phases I and II of the Protein Structure Initiative.  The MCSG Suite has been developed as a single, comprehensive set of crystallization conditions to be used in a standalone manner.

 

The end result is the best, first-pass screening suite possible, with:

  • data-driven selection individual conditions ensuring conditions with high productivity potential were not accidentally excluded
  • superior organization
  • the broadest coverage of crystallization space within a reasonable number of starting conditions for maximal efficiency while maintaining applicability for more unique and difficult targets.

 

"The Almo lab handles crystallization trials for the New York Structural Genomics Research Consortium (NYSGRC, part of PSI-Biology), the Enzyme Function Initiative (EFI) as well as serves as a core facility to the Einstein community and beyond. For all of these projects we have adopted the MCSG screens as our initial screening regiment. The 384-condition screening suite covers a broad spectrum of chemical space without redundancies and has proven to be very effective in producing diffraction quality crystals, frequently without the need for further optimization. We have also found these screens have proven to be very cost effective in comparison to other commercially available screens.” ~ Dr. Rafael Toro, Manager of Crystallization Operations , Almo Laboratory , AECOM"

MCSGScreens
SKU
MCSG-1

96 conditions, 1.7 mL each in a 96-well 2 mL deep well block
MCSG-1 $230.00
MCSG-1T

96 Conditions, 10 mL each in 15 mL conical tubes

MCSG-1T $600.00
MCSG-2

96 conditions, 1.7 mL each in a 96-well 2 mL deep well block
MCSG-2 $230.00
MCSG-2T

96 Conditions, 10 mL each in 15 mL conical tubes

MCSG-2T $600.00
MCSG-3

96 conditions, 1.7 mL each in a 96-well 2 mL deep well block
MCSG-3 $230.00
MCSG-3T

96 Conditions, 10 mL each in 15 mL conical tubes 

MCSG-3T $600.00
MCSG-4

96 conditions, 1.7 mL each in a 96-well 2 mL deep well block
MCSG-4 $230.00
MCSG-4T

96 Conditions, 10 mL each in 15 mL conical tubes 

MCSG-4T $600.00
Well Condition Suite # Volume Sell price Add to cart form
MCSG1-1 A1 1 MCSG-1 100mL $83.00
MCSG1-10 A10 10 MCSG-1 100mL $83.00
MCSG1-11 A11 11 MCSG-1 100mL $83.00
MCSG1-12 A12 12 MCSG-1 100mL $83.00
MCSG1-13 B1 13 MCSG-1 100mL $83.00
MCSG1-14 B2 14 MCSG-1 100mL $83.00
MCSG1-15 B3 15 MCSG-1 100mL $83.00
MCSG1-16 B4 16 MCSG-1 100mL $83.00
MCSG1-17 B5 17 MCSG-1 100mL $83.00
MCSG1-18 B6 18 MCSG-1 100mL $83.00
MCSG1-19 B7 19 MCSG-1 100mL $83.00
MCSG1-2 A2 2 MCSG-1 100mL $83.00
MCSG1-20 B8 20 MCSG-1 100mL $83.00
MCSG1-21 B9 21 MCSG-1 100mL $83.00
MCSG1-22 B10 22 MCSG-1 100mL $83.00
MCSG1-23 B11 23 MCSG-1 100mL $83.00
MCSG1-24 B12 24 MCSG-1 100mL $83.00
MCSG1-25 C1 25 MCSG-1 100mL $83.00
MCSG1-26 C2 26 MCSG-1 100mL $83.00
MCSG1-27 C3 27 MCSG-1 100mL $83.00

How was the MCSG screen developed?

The MCSG Crystallization Suite has been developed by the Midwest Center for Structural Genomics for use throughout the PSI:Biology initiative. The component crystallization conditions of the Suite have been mined from over a dozen of commercially available screens throughout 10 years of extensive crystallization trials carried out through previous phases of the PSI. Only conditions giving crystal hits were included in the screen, resulting in the most validated, chemically diverse and non-redundant screen on the market today.

What target type of targets is MCSG screen suitable for?

Over 40,000 targets from more than a 100 different organisms, both prokatyotic and eukaryotic, including bacteria, C. elegans, S. cerevisiae, humans and mice were used for data mining of the screen. The broadest target base of MCSG likely produced conditions that are more applicable to a larger number of protein families and organisms than any other sparse matrix screen on the market.

How is the screen organized?

The complete MCSG suite comprises 384 conditions. Conditions 1-288 are organized in order of decreasing crystal productivity in screens MCSG-1, MCSG-2 and MCSG-3. Thus MCSG-1 condition A1 has yielded the greatest number of unique crystals for all targets screened with the suite predecessors. The conditions that comprise MCSG-4 have been rationally selected to systematically sample regions of crystallization space for proteins requiring additional screening. They were selected from a set of conditions for crystallizing difficult proteins, with all conditions giving crystals.

I have a challenging target with not enough protein to run all four MCSG screens. Which of the MCSG screens should I chose to maximize the crystallization potential?

While the first three screens are arranged in order of productivity, MCSG 4 set was mined from singleton hit conditions of fewer difficult targets, and contains a more unique set of precipitants combinations. Therefore, running MCSG 1 and 2 would cover most productive conditions. Given enough protein, addition of MCSG4 will provide additional coverage of crystallization space.

Our lab stocks a variety of screens from multiple manufacturers, why should we consider adding yet another screen to our inventory?

With numerous crystallization screens available, it can be difficult to ensure that purchased screens do not overlap significantly with one another, sampling the same or very similar conditions multiple times. Setting up numerous screens for each sample is also costly in terms of consumption of precious protein, materials and time. Moreover, multiple survey studies indicate that 200-400 of well-selected conditions are sufficient to assess crystallizability of a protein sample. MCSG screen allows to streamline the screening process by covering the most chemical space in the least number of well-selected and validated conditions. Multiple structural genomics centers and industrial groups have adopted MCSG screen as the only initial screen, and are getting excellent result with it. For success stories please click here.

We use JCSG + or JCSG Core suite in our lab as a first screening pass, how is MCSG different?

The JCSG+ screen was mined from a limited set of Thermotoga maritima proteins, while JCSG Core suite was validated on a more diverse set of mostly bacterial targets. In contracst, MCSG was validated on the largest and most diverse set of organisms of any screen: over 40,000 tragets from over 100 prokatyotic and eukaryotic organisms. Therefore, MCSG conditions are more likely to be applicable to crystallization of increasingly more challenging targets from diverse organisms. Furthermore, MCSG screen was mined from a larger sampling of commercially available screens, ensuring greater coverage of crystallization space, and contains a wider variety of chemicals than JCSG screen. MCSG screen is chemically dissimilar to the JCSG screen based on the C6 analysis. While most conditions of the “core 67” JCSG+ set (conditions deemed critical for protein crystallization) were also found in the MCSG suite in identical or modified form, only 13 of these fell into the most productive MCSG1 set, meaning that MCSG1 contains a multitude of more productive conditions than JCSG+.

How chemically similar is MCSG to other sparse matrix screens on the market?

The analysis of MCSG screens using C6 server (http://c6.csiro.au/) revealed that overall the MCSG suite conditions are significantly different from the compositions of other commercial screens. MCSG4 is the only MCSG screen to exhibit significant overlap with another commercial screen, PEGsII suite from Qiagen. This is not surprising, given the consistent sampling of polyethylene glycols employed in the rationally designed MCS4 suite. However, given that the MCSG4 screen has been assembled to specifically complement the MCSG1-3 screens, we recommend its use as part of the MCSG paradigm to ensure that the subtleties of the full suite are not missed. In addition, MCSG suite exhibits an internal chemical diversity on par with other sparse matrix screens on the market.

What formats does the MCSG screen come in?

MCSG screen is available in the2 mL deep well block format with 1.7 mL of each condition. The blocks are heat-sealed for safer transportation and longer storage. The tube format consists of 15 mL comical tubes containing 10 mL of screening solutions. The tubes racks are shrink-wrapped for safer transportation.

How does the cost of MCSG screen compare to other screens on the market?

We provide the most competitive pricing of the screens in deep well block format due to the higher volume of solutions in each well (1.7 mL compared to 1.0 or 1.5 mL for most other manufacturers). Our tube format is competitively priced compared to most other manufacturers. MCSG can provide significant cost savings be virtue of being a very economical screen on per mL basis, and in addition it can be used to streamline your initial screening pipeline from multiple screens to just four.

Do you have production reports available?

Yes, Lot-specific production reports are available for the MCSG screen. Simply provide the Lot # from your block or tube set to support@microlytic.com and you will receive a detailed production report including the lots of the individual chemicals used within 24-48 hours.

Can I buy optimization reagents for the MCSG screen?

MCSG condition refills are available in 100 mL volumes. We also offer all individual component chemicals as liquid or dry stocks. These reagents are same high purity grade used in the MCSG screen manufacturing.