Designed for use with the Crystal Former, the SuperCOMBI screen offers efficient crystallization screening for liquid-liquid diffusion without the redundancy present in sparse matrix screens designed for vapor diffusion methods.
- 96 individual crystallization conditions
- Comprised of both MCSG and PurePEG screens.
- Efficient, non-redundant conditions designed to maximize the potential of capillary crystallization
- Available as deep-well block format with 1mL per condition
The SuperCOMBI has been design to maximize crystallization returns in the Crystal Former by creating a minimum set of conditions that, when coupled with the gradients sampled in the microcapillaries of the Crystal Former, provide broad and complete coverage of crysatllization space. The first 48 conditions derive from the MCSG crysatllization suite to provide high productivity in a pared down set of starting conditions. These conditions are among the most productive of those commercially available. Conditions 49-96 derive from the PurePEGs screen for which the Crystal Former is able to generate a complex PEG gradient to sample this commonly-successful region of crystallization space in an efficient and cost-effective manner.
Why diffusive mixing?
Diffusive mixing offers tremendous advantages in protein crystallization, including the ability to assess protein phase behavior across the gradient of a given condition for each microchannel used. In contrast to vapor diffusion, exploration of crystallization space becomes more systematic and complete in this format.
The Crystal Former can be readily automated on standard liquid handling robots for both protein and reagent dispenses. We have opted to include a 96-well Greiner source plate for customers wishing to use Mosquito (TTP LabTech) or NT8 (Formluatrix) to dispense the SuperCOMBI screen into the Crystal Formers. Users with systems from Art Robbins Instruments can source directly from the provided deep well block. For more information on appropriate protocols for automated Crystal Former set-up, please contact our support team.
|SSC-1 0.1 M sodium chloride, 0.1 M Bis-Tris:HCl 6.5, 1.5 M Ammonium Sulfate||SSC-1||$83.00|
|SSC-10 0.1 M Na2HPO4:Citric acid pH 4.2, 40% (v/v) PEG 300||SSC-10||$83.00|
|SSC-11 0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate:HCl pH 6.5, 0.4 PEG 300||SSC-11||$83.00|
|SSC-12 0.2 M ammonium sulfate, 0.1 M Bis-Tris:HCl pH 5.5, 25% (w/v) PEG 3350||SSC-12||$83.00|
|SSC-13 0.2 M magnesium chloride, 0.1 M HEPES:NaOH pH 7.5, 25% (w/v) PEG 3350||SSC-13||$83.00|
|SSC-14 0.2 M sodium chloride, 0.1 M Bis-Tris:HCl pH 5.5, 25% (w/v) PEG 3350||SSC-14||$83.00|
|SSC-15 0.2 M ammonium acetate, 0.1 M Bis-Tris:HCl pH 5.5, 25% (w/v) PEG 3350||SSC-15||$83.00|
|SSC-16 0.2 M magnesium chloride, 0.1 M Bis-Tris:HCl pH 6.5, 25% (w/v) PEG 3350||SSC-16||$83.00|
|SSC-17 0.2 M magnesium chloride, 0.1 M Tris:HCl pH 8.5, 25% (w/v) PEG 3350||SSC-17||$83.00|
|SSC-18 0.2 M sodium thiocyanate pH 6.9, 20% (w/v) PEG 3350||SSC-18||$83.00|
|SSC-19 0.2 M potassium thiocyanate, 20% (w/v) PEG 3350||SSC-19||$83.00|
|SSC-2 0.2 M ammonium phosphate monobasic, 0.1M Tris:HCl pH 8.5, 50% (v/v) MPD||SSC-2||$83.00|
|SSC-20 0.2 M potassium sodium tartrate, 20% (w/v) PEG 3350||SSC-20||$83.00|
|SSC-21 0.1 M Tris:HCl pH 8.5, 25% (w/v) PEG 3350||SSC-21||$83.00|
|SSC-22 0.2 M potassium iodide, 20% (w/v) PEG 3350||SSC-22||$83.00|