Our microfluidic crystallization devices have produces crystals for a number of exciting projects.  As sampling of these are presented here. 

 

 

With the ever-increasing threat posed by drug-resistant bacteria to public health, the search for new antibacterial therapies is an urgent one. Bacterial RNA polymerase (RNAP) is a well-established target for such broad-spectrum antibacterial therapy. Drugs of the rifamycin family act via RNAP inhibition and have been important therapies in the... more

Crystal Structure of an ABC transporter, substrate-binding protein from Brucella melitensis 16M in complex with L-Leucine using a crystal grown in a Crystal Former (Microlytic)

 

Crystal were obtained from SuoerCOMBI well B4: 0.2 M Magnesium Chloride, 0.1 M Bis-Tris: HCl, 25% PEG 3350 at 294 K. Crystals were harvested directly for in-house... more

Arna is a bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-... more

These images were kindly provided by Dr. Isabel Garcia-Saez at IBS-France in 2010.

,

images kindly provided by Dr. Robert Suto from his trials with the Crystal Former in 2010.

Crystallization examples for targets in the SSGCID crystallization pipeline screened in the SBS High Throughput Crystal Formers with the SuperCOMBI crystallization screen. These images have been produced with Federal funds from the National Institute of Allergy an Infectous Diseases, National institutes of Health, Department of Health and Human Services... more

,

"Endoglycosidase S (EndoS) is an enzyme secreted by Streptococcus pyogenes that specifically hydrolyzes the b-1,4-di-N-acetylchitobiose core glycan on immunoglobulin G (IgG) antibodies. One of the most common human pathogens and the cause of group A streptococcal infections, S. pyogenes secretes EndoS in order to evade the host immune system by rendering... more

Recent tests of the Original Crystal Formers with the Echo acoustic liquid handler from Labcyte (http://www.labcyte.com/Protein_Crystallography/Default.812.html) demonstrate that the precise loading capabilities of the robot permit complete loading of the Crystal Former channels with 150nL of protein solution (red and blue channels). This is the smallest... more

"AstraZeneca in Sweden have used the 16 channel Crystal Former scale-up chip for optimization of poorly-diffracting vapor diffusion hits. By using the compatible holders they set-up the chips with the TTP LabTech Mosquito robot and follow crystal growth with the RockImager system from Formulatrix.”  
Crystallization screening with the Crystal Former permits the exploration of a broad area of crystallization space. Owing to the gradients formed during liquid-liquid diffusion, it becomes possible to visualize both the precipitation and crystallization behavior of a protein along the established gradient.  Thus, a single crystallization trial can yield a... more

Pages