Crystal Former Publications


(13) Lee MJY, Faucher F & Jia Z (2014) growth of diffraction-quality protien crystals using a harvestable microfluidic device. Crystal Growth and Design. DOI:10.1021/cg500450b


(12) Degen D, Feng Y, Zhang Y, Ebright KY, Ebright YW, Gigliotti M, Vahedian-Movahed H, Mandal S, Talaue M, Connell N, Arnold E, Fenical W & Ebright RH. (2014) transcription inhibition by the depsipeptide antibiotic salinamide A. elifesciences. doi:


(12) Deller MC & RUpp B (2014) Approaches to automated protein crystal harvesting. Acta Cryst. F doi: 10.1107/S2053230X14000387


(11) Trastoy B, Lomino JV, Pierce BG, Carter LG, Gunther S, Giddens JP, Snyder GA, Weiss Tm, Weng Z, Wang LX & Sundberg EJ. (2014) Crystal structure fo Streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human antibodies. PNAS. doi: 10.1073/pnas.1322908111


(10) Khatter H, Myasnikov AG, Mastio L, Billas IML, birck C, Stella S, & Klaholz BP (2014). Purification, characterization and crystallization of the human 80S ribosome. Nucleic Acids Research. doi: 10.1093/nar/gkt1404


(9) Trastoy B, Lomino JV, Wang LX, & Sundberg EJ (2013). Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-beta-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG. Acta Crystallographica Section F: Structural Biology and Crystallization Communications doi:10.1107/S1744309113030650


(8) Sigdel M, Pusey ML, & Aygun RS (2013) Real-Time Protein Crystallization image acquisition and classification system. Crystal Growth & Design. 13(7), 2728-2736. DOI: 10.1021/cg3016029


(7) Manjasetty BA, Bussow K, Panjikar S & Turnbull AP (2012) Current methods in structural proteomics and its applications in biological sciences. 3BioTech,Springer Open DOI:10.1007/s13205-011-0037-1


(6) Hoffman ID (2012) Chapter 4: Protein crystallization for structure-based drug design. LTari LW(Ed). Structure-Based Drug Discovery, Methods in Molecular Biology 841. DOI: 10.1007/978-1-61779-520-6_4


(5) Garcia-Caballero A, Gavira JA, Pineda-Molina E, Chayen NE, Govada L, Khurshid S, Saridakis E, Boudjemline A, Swann MJ, Shaw Stewart P, Briggs RA, Kolek SA, Oberthuer D, Dierks K, Betzel C, Sanatana M, Hobbs JR, Thaw P, Savil TJ, Mesters JR, Hilgenfeld R, Bonander N, & Bill RM (2011) Optimization of Protein crystallization: The OptiCryst Project. Crystal Growth & Design 11, 2112-2121 DOI:10.1021/cg1013768


(4) Stojanoff V, Jakoncic J, Oren DA, Nagarajan V, Navarro Poulsen JC, Adams-Cioaba MA, Bergfors T & Sommer MOA (2011). From Screen to structure with a harvestable microfluidic device. Acta Cryst F67:971-975.  DOI:10.1107/S174430911102445


(3) Stewart PDS, Kolek SA, Briggs RA, Chayen NE & Baldock PFM (2011) Random microseeding: a theoretical and practical exploration of seed stability and seeding techniques for successful protein crystallization. Cryst. Growth Des. DOI: 10.1021/cg2001442.


(2) Gerdts  C * Nollert P (2009). Advances in microfluidic membrane protein crystallization techniques. Current Topics in Membranes, 63: 179-189. DOI: 10.1016/S1063-5823(09)63008-8


(1) Bergfors T (2009) The rapid crystallization strategy for structure-based inhibitor design. From molecules to medicines: Structure of biological macromolecules and its relevance in combating new diseases and bioterrorism. Sussman JL & Spadon P. Eds. ISBN 978-90-481-2337-7



Crystal Former Customer Conference Presentations


(2) Trastoy-Bello B & Sundberg E. (2014) Crystal structure of streptococcus pyogenes EndoS, an immunomodulatory endoglycosidase specific for human IgG antibodies.


(1) Weger A, Kim YC, Adams-Cioaba MA, Sommer M & Joachimiak A (2010) Microfluidic Crystal Formers for high throughput screening and optimization.  NIGMS Workshop: Enabling technologies in structure and function. View Here



MCSG Screen Publications


(26) Michalska K, Chhor G, Clancy S, Jedrzejczak R, Babnigg G, Winans SC & Joachimiak A (2014) RsaM- a transcriptional regulator of Burkholderia ssp. wiht novel fold. The FEBS journal. DOI: 10.111/febs.12868


(25) Do H, Kim IS, Kim YS, Shin SY,  Kim JJ, Mok JE, Park SI, Wi AR, Park H, Kim HW,  Yoon  HS &  Lee JH. (2014) Crystallization and preliminary x-ray crystallographic studies of dehydroascorbate reductase (DHAR) from Oryza sativa l. japonica. Acta Cryst. F. doi:10.1107/S2053230X14009133


(24) Amick J, Schlanger SE, Wachnowsky C, Moseng MA, Emerson CC, Dare M, Luo WI, Ithychanda SS, Nix JC, Cowan JA, Page RC & Misra S (2014) Crystal structure of the nucleotide binding domain of mortalin, the mitochondrial HSP70 chaperone. Protein Science. doi:10.1002/pro.2466


(23) Shrestha RK, Ronau JA, Davies CW, guenette RG, Strieter ER, Paul LN & Das C. (2014 Insights into the mechanism of deubiquitination by JAMM deubiquitinases from cocrystal structures of the enzyme wiht the substrate and product. Biochemistry. doi:10.1021/bi5003162


(22) Discovery of function in the enolase superfamily: D-mannonate and D-gluconate dehydratases in the D_mannonate dehydratase subgroup. Biochemistry doi:10.1012:bi600264p


(21) Erickson AI, Sarsam RD & Fisher AJ (2014) Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism. doi:10.1107/S2053230X14008619


(20) Do H, Kim SJ, Kim HJ & Lee JH (2014) Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1. Acta Cryst. D doi:10.1107/S1399004714000996


(19) Do H, Lee CW, Han SJ, Lee SG, Kim HJ, Park H & Lee JH (2014) Purification, crystallization and preliminary X-ray crystallographic studies of FMN-bound and FMN-free forms of aromatic acid decarboxylase 9CpsUbiX) from the psychrophilic bacterium Colwellia psychrerythraea 34H. Acta Cryst. F doi:10.1107/S2053230X1303447X


(18) Soniat M, Sampathkumar P, Collett G, Gizzi, AS, Banu RN, Bhosle RC, ... & Almo SC (2013). Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. Journal of Structural and Functional Genomics, doi:10.1007/s10969-013-9150-1 


(17) Kim, Y., Cunningham, M. A., Mire, J., Tesar, C., Sacchettini, J., & Joachimiak, A. (2013). NDM-1, the ultimate promiscuous enzyme: substrate recognition and catalytic mechanism. The FASEB Journal, 27(5), 1917-1927. doi:10.1096/fj.12-224014


(16) Pokkuluri PR, Dwulit-Smith J, Duke NE, Wilton R, Mack JC, Bearden, J, ... & Schiffer M (2013). Analysis of periplasmic sensor domains fromAnaeromyxobacter dehalogenans 2CP-C: Structure of one sensor domain from a histidine kinase and another from a chemotaxis protein.MicrobiologyOpen. doi:10.1002/mbo3.112


(15) Lohman JR, Ma M, Cuff ME, Bigelow L, Bearden J, Babnigg G, ... & Shen B (2013). The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins. Proteins: Structure, Function, and Bioinformatics. doi: 10.1002/prot.24485 


(14) Michalska K, Brown RN, Li H, Jedrzejczak R, Niemann GS, Heffron F, ... & Joachimiak A. (2013). New sub-family of lysozyme-like proteins shows no catalytic activity: crystallographic and biochemical study of STM3605 protein from Salmonella Typhimurium. Journal of Structural and Functional Genomics, 1-10. doi:10.1007/s10969-013-9151-0


(13) Lee S, Kim MK, Ji CJ, Lee JW, & Cha SS. (2013). Experimental phasing using zinc and sulfur anomalous signals measured at the zinc absorption peak. Journal of Microbiology, 51(5), 639-643.doi:10.1007/s12275-013-3412-2


(12) Shnitsar V, Li J, Li X, Calmettes C, Basu A, Casey JR, ... & Reithmeier RA. (2013). A Substrate Access Tunnel in the Cytosolic Domain Is Not an Essential Feature of the Solute Carrier 4 (SLC4) Family of Bicarbonate Transporters. Journal of Biological Chemistry. doi:10.1074/jbc.M113.511865 


(11) Ali, S. S., Whitney, J. C., Stevenson, J., Robinson, H., Howell, P. L., & Navarre, W. W. (2013). Structural Insights into the Regulation of Foreign Genes in Salmonella by the Hha/H-NS Complex. Journal of Biological Chemistry,288(19), 13356-13369. doi:10.1074/jbc.M113.455378


(10) Tan, K., Chang, C., Cuff, M., Osipiuk, J., Landorf, E., Mack, J. C., ... & Collart, F. R. (2013). Structural and functional characterization of solute binding proteins for aromatic compounds derived from lignin: p‐coumaric acid and related aromatic acids. Proteins: Structure, Function, and Bioinformatics. doi: 10.1002/prot.24305


(9) Michalska, K., Tan, K., Li, H., Hatzos-Skintges, C., Bearden, J., Babnigg, G., & Joachimiak, A. (2013). GH1-family 6-P--glucosidases from human microbiome lactic acid bacteria. Acta Crystallographica Section D: Biological Crystallography, 69(3), 451-463. doi: 10.1107/S0907444912049608


(8) Koellhoffer, J. F., Dai, Z., Malashkevich, V. N., Stenglein, M. D., Liu, Y., Toro, R., ... & Lai, J. R. (2013). Structural Characterization of the glycoprotein GP2 Core Domain from the< i> CAS Virus</i>, a Novel Arenavirus-like Species.Journal of Molecular Biology. doi: 10.1016/j.jmb.2013.12.009


(7) Wu, R., Gu, M., Wilton, R., Babnigg, G., Kim, Y., Pokkuluri, P. R., ... & Schiffer, M. (2013). Insight into the sporulation phosphorelay: Crystal structure of the sensor domain of Bacillus subtilis histidine kinase, KinD. Protein Science. doi: 10.1002/pro.2237


(6) Poukchanski A, Fritz HM, Tonkin ML, Treeck M, Boulanger MJ, & Boothroyd JC (2013). Toxoplasma gondii Sporozoites Invade Host Cells Using Two Novel Paralogues of RON2 and AMA1. PLoS One. doi: 10.1371/journal.pone.0070637 


(5) Michalska K, Chang C, Mack JC, Zerbs S, Joachimiak A, & Collart FR (2012) Characterization of transport proteins for aromatic compounts derived from Lignin: Benzoate derivative binding proteins. Jounral of Molecular Biology doi:10/1016/j.jmb.2012.08.017.


(4) Koellhoffer JF, Malashkevich VN, Harrison JS, Toro R, Bhosle RC, Chandran K, Almo SC, Lai JR (2012) Crystal structure of the Marburg Virus GP2 core domain in its postfusion conformation. Biochemistry. doi: 10.1021/bi300976m.


(3) Makowska-Grzyska, Kim Y, Wu R, Wilton R, Gollapalli DR, Wang XK, Zhang R, Jedrzejczak R, Mack JC, Maltseva N, Mulligan R, Binkowski A, Gornicki P, Kuhn ML, Anderson WF, Hedstrom L and Joachimiak A (2012)Bacillus antracis Inosine 5'-monophosphate dehydrogenase in action: the first bacterial series of structures of phosphate ion-, substrate- and product=bound complexes. Biochemistry, 51(31): 6148-6163.doi:10.1021/bi300511w


(2) Kim Y, Tesar C, Mire J, Jedrzejczak R, Binkowski A, Babnigg G, Sacchettini J, and Joachimiak A (2011) Structure of apo- and monometalated forms of NDM-1 - a highly potent carbapenem-hydrolyzing metallo-beta-lactamase. PloS ONE 6(9): e24621. doi:10.1371/journal.pone.0024621


(1) Kim Y, Babnigg G, Jedrzejczak R, Eschenfeldt WH, Li H, Maltseva N, Hatzos-Skintges C, Gu M, Makowska-Grzyska M, Wu R, An H, Chhor G and Joachimiak A. (2011) High-throughput protein purification and quality assessment for crystallization. Methods 55: 12-28. doi:10.1016/j.ymeth.2011.07.010